Toxicology testing is performed in clinical settings, forensic settings, and for controlling doping.Drug screening is a toxicology test to determine if drugs are present in biological samples.The most common specimen type for drug testing is urine, as drugs and/or their metabolites are often more concentrated in the urine, extending the detection window of drugs.The dilute-and-shoot method is a simple procedure used in toxicology testing, where a sample is diluted before being directly injected into the NEFF I38KHV2S0 N90 Built In 37cm 3 Speeds Island Cooker Hood Black liquid chromatography-mass spectrometry (LC-MS) system.
This method is easy, quick, and cost-saving, and can be used for protein-poor liquid specimens such as urine.Thus, it is reasonable and attractive for busy toxicology laboratories to combine the dilute-and-shoot method with high-resolution hyphenated-MS for urine drug screening.This method has several disadvantages, including a suboptimal detection capability for certain analytes, as well as interference from co-eluting matrix components called matrix effects, in which co-eluting matrix molecules alter the ionization efficiency of the analyte molecules at the ionization source in LC-MS, altering (mostly reducing) the analyte detection capability.The matrix effect testing is essential for the Home Decor validation of LC-MS-based assays.
A reasonable approach to addressing these undesirable effects would be to minimize these components.The most straightforward approach is to reduce the amounts of matrix components by using a higher dilution of the specimen and a lower volume for specimen injection.Optimization of the chromatographic separation is another reasonable approach for reducing co-eluting matrix components with the analyte.